Journal: The Journal of Neuroscience

Article Title: The Role of CREB, SRF, and MEF2 in Activity-Dependent Neuronal Plasticity in the Visual Cortex

doi: 10.1523/JNEUROSCI.0766-17.2017

Figure Lengend Snippet: Primary and secondary antibodies used for Western blotting

Article Snippet: Western blots for the developmental time points were performed on naive mice using similar conditions as described above by the University of Maryland School of Medicine Center for Innovative Biomedical Resources. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody name Dilution Species source Company RRID Phospho-CREB (Ser133) 1:1000 Rabbit Millipore Catalog #06-519 AB_310153 CREB 1:5000 Rabbit Millipore Catalog #04-218 AB_1586958 Egr1 1:1000 Rabbit Santa Cruz Biotechnology Catalog #sc-110 AB_2097174 Ube3a 1:1000 Rabbit Cell Signaling Technology Catalog #7526S AB_10971637 SRF 1:1000 Mouse Santa Cruz Biotechnology Catalog #sc-25290 AB_2239787 MEF2 1:1000 Goat Santa Cruz Biotechnology Catalog #sc-13917 AB_2142728 Cyclophilin B 1:10,000 Rabbit Thermo Fisher Scientific Catalog #PA1-027A AB_2169138 Anti-rabbit IgG 1:3000 Goat Cell Signaling Technology Catalog #7074 AB_2099233 Anti-mouse IgG 1:3000 Goat Cell Signaling Technology Catalog #7076 AB_330924 Anti-goat IgG 1:3000 Rabbit KPL Catalog #14-13-06 Open in a separate window This table contains a list of all primary (rows 1–7) and secondary (rows 8–10) antibodies used for Western blotting, along with specifications like concentration used, species source, company name, product number, and RRID.

Techniques: Western Blot

Journal: The EMBO Journal

Article Title: Hypoxia promotes tumor immune evasion by suppressing MHC-I expression and antigen presentation

doi: 10.1038/s44318-024-00319-7

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Anti-rabbit IgG HRP-linked , Merck , 31460.

Techniques: Recombinant, Western Blot, SYBR Green Assay, Plasmid Preparation, Software

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Sodium-calcium exchanger complexed with GM1 ganglioside in nuclear membrane transfers calcium from nucleoplasm to endoplasmic reticulum

doi: 10.1073/pnas.0903408106

Figure Lengend Snippet: Expression of NCX and GM1. (A) Nuclei of NG108–15 (differentiated), NG-CR72, C6, and Jurkat cells were isolated, and fixed. GM1 and NCX in the NE were revealed by double staining with CtxB-FITC and anti-NCX Ab (second Ab-linked to Texas red). Images show coexpression of NCX and GM1 in the NE of NG108–15 and C6 cells; NG-CR72 cells showed NCX but no GM1 while Jurkat cells showed GM1 but no NCX. (B) NCX in NE and non-nuclear membrane mixture (M. Mix.) was immunoprecipitated with mouse anti-NCX mAb (C2C12), subjected to SDS/PAGE and electrophoretic transfer to PVDF; it was then blotted with rabbit polyclonal anti-NCX Ab (a). NG108–15 and NG-CR72 cells gave bands corresponding to known NCX pattern in the NE and non-nuclear M. Mix. (e.g., plasma membrane) while C6 cells expressed NCX only in the NE. Jurkat cells were devoid of NCX in both fractions. Parallel blot using CtxB-HRP (b) revealed tight association of GM1 with major NCX bands in the NE of NG108–15 and C6 cells, but not GM1-deficient NG-CR72 cells or NCX-deficient Jurkat cells.

Article Snippet: The proteins were electrophoretically transferred to a PVDF membrane and blotted with polyclonal rabbit anti-NCX Ab (Swant Swiss Antibodies) plus goat anti-rabbit IgG Ab linked to HRP; a parallel gel was blotted with cholera toxin B-HRP for GM1.

Techniques: Expressing, Isolation, Double Staining, Membrane, Immunoprecipitation, SDS Page, Clinical Proteomics

Journal: eLife

Article Title: TALE factors use two distinct functional modes to control an essential zebrafish gene expression program

doi: 10.7554/eLife.36144

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-rabbit IgG, HRP-linked secondary antibody , Jackson Laboratories , 211-032-171 , RRID: AB_2339149.

Techniques: Control, Subcloning, Recombinant, Sample Prep, Mutagenesis, Knockdown, Methylated DNA Immunoprecipitation Sequencing, Plasmid Preparation, Software